Design of a selective chemical probe for class I PDZ domains.
نویسندگان
چکیده
Covalent labeling has been widely used for structural and functional analyses of proteins. To target a wide range of PDZ domains, we designed a chemical scaffold mimicking the E/D-T/S-XV peptide, which is a PDZ domain that binds ligands in higher occurrence. A chemical probe (2) that contained this moiety alkylated diverse PDZ domains, including NHERF-1 PDZ2, and differentially visualized the cellular proteome.
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ورودعنوان ژورنال:
- Bioorganic & medicinal chemistry letters
دوره 17 2 شماره
صفحات -
تاریخ انتشار 2007